电离辐射通过转化生长因子-β-介导的上皮-上皮细胞转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：探讨紫外线是不是可通过转化介素-β（TGF-β）-诱导的视网膜-间质叠加 (EMT)来促进免疫细胞的侵袭迁到。使用据统计2Gy(60)Coγ线强光源自本能器官的6种免疫细胞，纪录与EMT相关的叠加，这包括分别利用电子显微镜技术，亚基质印迹分析方法，免疫荧光技术，划痕测试和Transwell的大测试来辨别并样品细胞组织形态，EMT记号，侵袭迁到意志力等。改用激酶嗣后免疫吸附法样品这些免疫细胞里TGF-β亚基技术水平，利用比如说抑制剂SB431542来评估TGF-β信号通路在紫外线EMT里的发挥作用。经过据统计为2Gy强光的免疫细胞里存有间叶细胞的表达，与假强光组比起其视网膜记号减少，间叶细胞记号增高，同时其侵袭移往意志力大幅提高，TGF-β亚基技术水平也提高。促使见到由A549紫外线诱导的EMT可通过对TGF-β信号抑制发生关键时刻。这些结果表明TGF-β诱导的EMT在紫外线诱导大幅提高免疫细胞侵袭移往意志力里起着起着。

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