电离辐射通过转化生长因子-β-介导的上皮-间质切换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

参考资料 ：探讨放射是否可通过转化生长因子-β（TGF-β）-细胞内内的视网膜-间质转换 (EMT)来增进癌细胞内的首当其冲迁离。使用工业产值2Gy(60)Coγ线照射源自生物人体器官的6种癌细胞内，就有与EMT特别的变化，这包括分别利用显微镜技术，酶质索科利夫卡工具，免疫荧光技术，划痕次测试和Transwell小室次测试来观察并检查细胞内组成员织形态，EMT标记，首当其冲迁离能力等。引入酶联免疫吸附通则检查这些癌细胞内当中TGF-β酶高水平，利用特别肽SB431542来评估TGF-β信号自营在放射EMT当中的作用。经过工业产值为2Gy照射的癌细胞内当中存在间叶细胞内的理解，与；也照射组成员相对其视网膜标记减缓，间叶细胞内标记增加，同时其首当其冲转回能力强化，TGF-β酶高水平也大大提高。进一步发现由A549放射其会的EMT可通过对TGF-β信号抑制频发逆转。这些结果表明TGF-β细胞内内的EMT在放射其会强化癌细胞内首当其冲转回能力当中起着关键作用。

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